BigIP exhibiting TCP zero Window behavior and closing connection

have successfully implemented HA configuration on numerous f5 LTM unit across our network without encountering any issue. Specifically, we are wondering why only one application sems to be impacted after the trunk configuration in f5 LTM.

As of now, the application is functioning smoothly with standard VIP, and there have been no reported related to download speed or performance and currnely physical interface direclty map to VLAN. However, we are keen to understand why this specific application is experiencing difficulties following trunk configuration.