iRule Forward client traffic to different port by source IP addresses

No, you can't use HTTP_REQUEST if you are not offloading SSL, but you can use CLIENT_ACCEPTED and source ip to determine the pool member (or port).

You can use either the node command or the pool command to specify the serverside connection:

https://devcentral.f5.com/wiki/iRules.node.ashx https://devcentral.f5.com/wiki/iRules.pool.ashx